Human HAI-1 Protein (HAI-HM11A)
Human HAI-1 Protein (HAI-HM11A)
Human HAI-1 Protein (HAI-HM11A)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly36-Val433
Catalog #HAI-HM11A
Regular price $315.00
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Product Description

Recombinant Human HAI-1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly36-Val433.

Product Background

SPINT1, also known as HAI-1, is a Kunitz-type serine protease inhibitor that inhibits multiple proteases including hepatocyte growth factor (HGF) activator and matriptase. SPINT1 has been shown to modulate HGF/MET activation in certain cancer types.

Product Category

Recombinant Protein / Antibody discovery

Protein

HAI-1

Synonyms

HAI 1; SPINT1; HAI1; HAI-1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gly36-Val433

Molecular Weight

The protein has a predicted MW of 45.50 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human HAI-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HAI-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2. The IC50 is < 1 nM. Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2. The IC50 is < 1 nM.
The purity of Human HAI-1 is greater than 95% as determined by SEC-HPLC. The purity of Human HAI-1 is greater than 95% as determined by SEC-HPLC.

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