Human IL-17F Protein (ILF-HM119)
Human IL-17F Protein (ILF-HM119)
Human IL-17F Protein (ILF-HM119)
Human IL-17F Protein (ILF-HM119)
Human IL-17F Protein (ILF-HM119)
Human IL-17F Protein (ILF-HM119)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17 R alpha, hFc Tag with the EC50 of 0.63ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg31-Gln163
Catalog #ILF-HM119
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Product Description
 Recombinant Human IL-17F Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg31-Gln163.
Product Background
 The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus.IL-17F is ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover.
Product Category
 Recombinant Protein
Target
 IL-17F
Synonyms
 Cytokine ML-1; IL17F; IL-17F; IL24; IL-24; CANDF6; IL-17F; ML1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Arg31-Gln163
Molecular Weight
 The protein has a predicted MW of 15.7 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-17F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17 R alpha, hFc Tag with the EC50 of 0.63ug/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-17F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17 R alpha, hFc Tag with the EC50 of 0.63ug/ml determined by ELISA.
Human IL-17F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-17F is greater than 95% as determined by SEC-HPLC.
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