• Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized Human R-Spondin 3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LGR-4 Nanodisc, His Tag with the EC50 of 0.36ug/ml determined by ELISA (QC Test). The affinity constant of 0.40 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Met1-Asp951
Catalog #LGR-HM10N
Price
Regular price $1,21000
Regular price Sale price $1,21000
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Size
Avantor VWR Fisher Scientific

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Human LGR-4 Nanodisc (LGR-HM10N) Summary

Product Description

 Recombinant Human LGR-4 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Asp951.

Product Background

 Leucine-rich repeat-containing G protein-coupled receptor (LGR)-4 is a G protein-coupled receptor (GPCR) with a seven-transmembrane domain structure. LGRs are evolutionally and structurally phylogenetic, classified into three subgroups and are members of the so-called orphan receptors.

Human LGR-4 Nanodisc (LGR-HM10N) Specifications/Details

Protein
 LGR-4
Synonyms
 LGR4; GPR48
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met1-Asp951
Molecular Weight
 The protein has a predicted MW of 105.80 kDa.
Activity
 Immobilized Human R-Spondin 3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LGR-4 Nanodisc, His Tag with the EC50 of 0.36ug/ml determined by ELISA (QC Test). The affinity constant of 0.40 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human R Spondin 3, His Tag immobilized on CM5 Chip can bind Human LGR-4 Nanodisc, His Tag with an affinity constant of 0.40 nM as determined in SPR assay (Biacore T200). Human R Spondin 3, His Tag immobilized on CM5 Chip can bind Human LGR-4 Nanodisc, His Tag with an affinity constant of 0.40 nM as determined in SPR assay (Biacore T200).
Immobilized Human R-Spondin 3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LGR-4 Nanodisc, His Tag with the EC50 of 0.36ug/ml determined by ELISA (QC Test). Immobilized Human R-Spondin 3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LGR-4 Nanodisc, His Tag with the EC50 of 0.36ug/ml determined by ELISA (QC Test).
Other Available Proteins:
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