• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Tyr22-Pro473
Catalog #AMC-MM101
Price
Regular price $26400
Regular price Sale price $26400
/
Size
Avantor VWR Fisher Scientific

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Mouse AMCase/CHIA Protein (AMC-MM101) Summary

Product Description

 Recombinant Mouse AMCase/CHIA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Pro473.

Product Background

 Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. The expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation.

Mouse AMCase/CHIA Protein (AMC-MM101) Specifications/Details

Protein
 AMCase/CHIA
Synonyms
 Acidic mammalian chitinase; AMCase; CHIA; CHIT2; EC 3.2.1.14; Chia1; YNL;EC3.2.1.14
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Tyr22-Pro473
Molecular Weight
 The protein has a predicted MW of 51 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse AMCase on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse AMCase on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse AMCase is greater than 95% as determined by SEC-HPLC. The purity of Mouse AMCase is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...