• Express System: HEK293
  • Product Tag: N-monomeric hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ala134-Leu285
Catalog #BAF-HM214
Price
Regular price $346.50
Regular price Sale price $346.50
/
Size
Avantor VWR Fisher Scientific

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Human BAFF/TNFSF13B/CD257 Protein (BAF-HM214) Summary

Product Description

 Recombinant Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with monomeric Fc tag at the N-Terminus.It contains Ala134-Leu285.

Product Background

 B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human BAFF/TNFSF13B/CD257 Protein (BAF-HM214) Specifications/Details

Protein
 BAFF/TNFSF13B/CD257
Synonyms
 BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala134-Leu285
Molecular Weight
 The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).
Product Tag
 N-monomeric hFc

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200). Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).
Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). Immobilized Human BCMA Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
Immobilized Human BAFF, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 1.7ng/ml determined by ELISA. Immobilized Human BAFF, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 1.7ng/ml determined by ELISA.
Immobilized Human BAFF, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BAFF Antibody, hFc Tag with the EC50 of 28.2ng/ml determined by ELISA. Immobilized Human BAFF, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BAFF Antibody, hFc Tag with the EC50 of 28.2ng/ml determined by ELISA.
Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human BAFF, monomeric hFc Tag on ProA-Biosensor can bind Human BCMA (Trimer), His Tag with an affinity constant of 0.36 nM as determined in BLI assay. Loaded Human BAFF, monomeric hFc Tag on ProA-Biosensor can bind Human BCMA (Trimer), His Tag with an affinity constant of 0.36 nM as determined in BLI assay.
Loaded Human BAFF, monomeric hFc Tag on ProA-Biosensor can bind Human BAFFR, His Tag with an affinity constant of 34.80 nM as determined in BLI assay. Loaded Human BAFF, monomeric hFc Tag on ProA-Biosensor can bind Human BAFFR, His Tag with an affinity constant of 34.80 nM as determined in BLI assay.
The purity of Human BAFF is greater than 90% as determined by SEC-HPLC. The purity of Human BAFF is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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