• Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human 4-1BB Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
  • Amino Acid Range: Arg71-Glu254
Catalog #BBL-HM141
Price
Regular price $346.50
Regular price Sale price $346.50
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Size
Avantor VWR Fisher Scientific

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Human 4-1BB Ligand/TNFSF9 Trimer Protein (BBL-HM141) Summary

Product Description

 Recombinant Human 4-1BB Ligand/TNFSF9 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Arg71-Glu254 trimer design.

Product Background

 The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.

Human 4-1BB Ligand/TNFSF9 Trimer Protein (BBL-HM141) Specifications/Details

Protein
 4-1BB Ligand/TNFSF9
Synonyms
 41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Arg71-Glu254
Molecular Weight
 The protein has a predicted MW of 61.8 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human 4-1BB Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Product Tag
 N-His-Flag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 300mM NaCL (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human 4-1BB Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. Immobilized Human 4-1BB Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Human 4‑1BB Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human 4‑1BB Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Non-biotinylated Human 4-1BB, hFc-Avi Tag on ProA-Biosensor can bind Human 4-1BB Ligand (Trimer), His Tag with an affinity constant of 0.18 nM as determined in BLI assay . Loaded Non-biotinylated Human 4-1BB, hFc-Avi Tag on ProA-Biosensor can bind Human 4-1BB Ligand (Trimer), His Tag with an affinity constant of 0.18 nM as determined in BLI assay .
The purity of-Human 4‑1BB Ligand Trimer is greater than 95% as determined by SEC-HPLC. The purity of-Human 4‑1BB Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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