• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its esterase activity. The specific activity is > 200 pmol/min/µg.
  • Amino Acid Range: Gln32-Asp390
Catalog #CA9-MM101
Price
Regular price $36300
Regular price Sale price $36300
/
Size
Avantor VWR Fisher Scientific

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Mouse CA9/Carbonic Anhydrase IX Protein (CA9-MM101) Summary

Product Description

 Recombinant Mouse CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Asp390.

Product Background

 CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.

Mouse CA9/Carbonic Anhydrase IX Protein (CA9-MM101) Specifications/Details

Protein
 CA9/Carbonic Anhydrase IX
Synonyms
 CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC;EC4.2.1.1
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Gln32-Asp390
Molecular Weight
 The protein has a predicted MW of 43.12 kDa. Due to glycosylation, the protein migrates to 50-53 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its esterase activity. The specific activity is > 200 pmol/min/µg.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CA9 is greater than 95% as determined by SEC-HPLC. The purity of Mouse CA9 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...