• Express System: E.coli
  • Product Tag: N-His,SUMO
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CCL5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
  • Amino Acid Range: Ser24-Ser91
Catalog #CCL-HE105
Price
Regular price $33000
Regular price Sale price $33000
/
Size
Avantor VWR Fisher Scientific

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Human CCL5 Protein (CCL-HE105) Summary

Product Description

 Recombinant Human CCL5 Protein is expressed from E.coli with His-SUMO tag at the N-Terminus.It contains Ser24-Ser91.

Product Background

 The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.

Human CCL5 Protein (CCL-HE105) Specifications/Details

Protein
 CCL5
Synonyms
 MuRantes; SIS-delta; Scya5; Ccl5; D17S136E; eoCP; RANTES; SCYA5; SISd; TCP228; CCL5
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser24-Ser91
Molecular Weight
 The protein has a predicted MW of 21 kDa. The protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CCL5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
Product Tag
 N-His,SUMO
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 90% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM Tris, 0.3M NaCl (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CCL5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. Immobilized Human CCL5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
Human CCL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Human CCL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Other Available Proteins:
Finding available proteins...