Human IgG1 Fc Protein (IGG-HM001)

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asp104-Lys330

Catalog: IGG-HM001

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Product Description	Recombinant Human IgG1 Fc is expressed from HEK293 without tag.It contains Asp104-Lys330.
Product Background	IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
Product Category	Recombinant Protein

Target	IgG1 Fc
Synonyms	IgG1; Igh-4; RGD1359539; VH7183
Accession	P01857-1
Species	Human
Amino Acid Range	Asp104-Lys330
Molecular Weight	The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 31-33 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).
	The purity of Human IgG1 Fc is greater than 95% as determined by SEC-HPLC.
	Human IgG1 Fc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.