Product Description |
Recombinant Human IgG1 Fc is expressed from HEK293 without tag.It contains Asp104-Lys330. |
Product Background |
IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases. |
Product Category |
Recombinant Protein |
Target |
IgG1 Fc |
Synonyms |
IgG1; Igh-4; RGD1359539; VH7183 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp104-Lys330 |
Molecular Weight |
The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 31-33 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). |
|
|
Human IgG1 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human IgG1 Fc is greater than 95% as determined by SEC-HPLC. |
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