• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human CD161, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD161 Antibody, hFc Tag with the EC50 of 39.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.65 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gln67-Ser225
Catalog #CD1-HM261
Price
Regular price $37950
Regular price Sale price $37950
/
Size
Avantor VWR Fisher Scientific

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Human CD161 Protein (CD1-HM261) Summary

Product Description

 Recombinant Human CD161 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln67-Ser225.

Product Background

 CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment.

Human CD161 Protein (CD1-HM261) Specifications/Details

Protein
 CD161
Synonyms
 KLRB1; CLEC5B; NKRP1A; NKR-P1A; HNKR-P1a; CD161; Ly59; NKR; NKRP1; NKR-P1; NKRP1ANKR
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln67-Ser225
Molecular Weight
 The protein has a predicted MW of 45.2 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD161, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD161 Antibody, hFc Tag with the EC50 of 39.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.65 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human CD161, hFc Tag immobilized on CM5 Chip can bind Human CLEC2D Tetramer, hFc Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200). Human CD161, hFc Tag immobilized on CM5 Chip can bind Human CLEC2D Tetramer, hFc Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200).
Immobilized Human CD161, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD161 Antibody, hFc Tag with the EC50 of 39.1ng/ml determined by ELISA (QC Test). Immobilized Human CD161, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD161 Antibody, hFc Tag with the EC50 of 39.1ng/ml determined by ELISA (QC Test).
Human CD161 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD161 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD161 is greater than 90% as determined by SEC-HPLC. The purity of Human CD161 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...