Mouse CD4/LEU3 Protein (CD4-MM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Lys27-Thr394

Catalog #CD4-MM101

Price

$264⁰⁰

Size

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Mouse CD4/LEU3 Protein (CD4-MM101) Summary

Product Description

Recombinant Mouse CD4/LEU3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys27-Thr394.

Product Background

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.

Mouse CD4/LEU3 Protein (CD4-MM101) Specifications/Details

Protein	CD4/LEU3
Synonyms	CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
Accession	NP_038516.1
Species	Mouse
Biotinylated	no
Amino Acid Range	Lys27-Thr394
Molecular Weight	The protein has a predicted MW of 42.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Mouse CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse CD4 is greater than 95% as determined by SEC-HPLC.