• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD83, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti- CD83 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
  • Amino Acid Range: Thr20-Glu147
Catalog #CD8-CM183
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus CD83 Protein (CD8-CM183) Summary

Product Description

 Recombinant Cynomolgus CD83 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Glu147.

Product Background

 CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.

Cynomolgus CD83 Protein (CD8-CM183) Specifications/Details

Protein
 CD83
Synonyms
 CD83; HB15; BL11; CD83 molecule
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Thr20-Glu147
Molecular Weight
 The protein has a predicted MW of 15.43 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus CD83, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti- CD83 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus CD83, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti- CD83 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA. Immobilized Cynomolgus CD83, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti- CD83 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
Cynomolgus CD83 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus CD83 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus CD83 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus CD83 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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