• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA.
  • Amino Acid Range: Leu23-Gly150
Catalog #CD8-HM18H
Price
Regular price $346.50
Regular price Sale price $346.50
/
Size
Avantor VWR Fisher Scientific

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Human CD28H/IGPR-1 Protein (CD8-HM18H) Summary

Product Description

 Recombinant Human CD28H/IGPR-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Gly150.

Product Background

 CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.

Human CD28H/IGPR-1 Protein (CD8-HM18H) Specifications/Details

Protein
 CD28H/IGPR-1
Synonyms
 TMIGD2; IGPR1; IGPR-1; CD28H; TPC1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu23-Gly150
Molecular Weight
 The protein has a predicted MW of 15.1 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA. Immobilized Human CD28H, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA.
Human CD28H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD28H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD28H is greater than 95% as determined by SEC-HPLC. The purity of Human CD28H is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...