• Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA.
  • Amino Acid Range: Met113-Leu261
Catalog #CDL-HM140
Price
Regular price $297.00
Regular price Sale price $297.00
/
Size
Avantor VWR Fisher Scientific

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Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (CDL-HM140) Summary

Product Description

 Recombinant Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Met113-Leu261 trimer design.

Product Background

 CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (CDL-HM140) Specifications/Details

Protein
 CD40 Ligand/TNFSF5
Synonyms
 CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Accession
Species
 Human/Cynomolgus/Rhesus macaque
Biotinylated
 no
Amino Acid Range
 Met113-Leu261
Molecular Weight
 The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA.
Product Tag
 N-His-Flag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA. Immobilized Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA.
Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human CD40, hFc Tag on ProA-Biosensor can bind Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), His-Flag Tag with an affinity constant of 0.23 µM as determined in BLI assay. Loaded Human CD40, hFc Tag on ProA-Biosensor can bind Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), His-Flag Tag with an affinity constant of 0.23 µM as determined in BLI assay.
The purity of Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC. The purity of Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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