• Express System: HEK293
  • Product Tag: N-monomeric hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA.
  • Amino Acid Range: Met112-Leu260
Catalog #CDL-MM240
Price
Regular price $28050
Regular price Sale price $28050
/
Size
Avantor VWR Fisher Scientific

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Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240) Summary

Product Description

 Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus; It contains Met112-Leu260 trimer design.

Product Background

 CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240) Specifications/Details

Protein
 CD40 Ligand/TNFSF5
Synonyms
 CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Met112-Leu260
Molecular Weight
 The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA.
Product Tag
 N-monomeric hFc
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 200 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA. Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA.
Mouse CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Mouse CD40, His Tag on Anti-His-Biosensor can bind Mouse CD40 Ligand (Trimer), monomeric hFc Tag with an affinity constant of 0.10 nM as determined in BLI assay . Loaded Mouse CD40, His Tag on Anti-His-Biosensor can bind Mouse CD40 Ligand (Trimer), monomeric hFc Tag with an affinity constant of 0.10 nM as determined in BLI assay .
The purity of Mouse CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC. The purity of Mouse CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...