• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus ACE2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
  • Amino Acid Range: Arg319-Phe541
Catalog #COV-VM2BD
Price
Regular price $363.00
Regular price Sale price $363.00
/
Size
Avantor VWR Fisher Scientific

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SARS-COV-2 Spike RBD Protein (COV-VM2BD) Summary

Product Description

 Recombinant SARS-COV-2 Spike RBD Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Arg319-Phe541.

Product Background

 The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

SARS-COV-2 Spike RBD Protein (COV-VM2BD) Specifications/Details

Protein
 Spike RBD
Synonyms
 S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession
Species
 SARS-COV-2
Biotinylated
 no
Amino Acid Range
 Arg319-Phe541
Molecular Weight
 The protein has a predicted MW of 50.7 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus ACE2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus ACE2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. Immobilized Cynomolgus ACE2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
SARS-COV-2 Spike RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS-COV-2 Spike RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC. The purity of SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...