• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2ug/ml (100ul/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.
  • Amino Acid Range: Gln14-Arg683(D614G)
Catalog #COV-VM4SG
Price
Regular price $36300
Regular price Sale price $36300
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated SARS-COV-2 Spike S1 (D614G) Protein (COV-VM4SG) Summary

Product Description

 Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683(D614G).

Product Background

 The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.

Non-biotinylated SARS-COV-2 Spike S1 (D614G) Protein (COV-VM4SG) Specifications/Details

Protein
 Spike S1
Synonyms
 S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Accession
Species
 SARS-COV-2
Biotinylated
 no
Amino Acid Range
 Gln14-Arg683(D614G)
Molecular Weight
 The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2ug/ml (100ul/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2ug/ml (100ul/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA. Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2ug/ml (100ul/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.
SARS-COV-2 Spike S1 (D614G) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS-COV-2 Spike S1 (D614G) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike S1 (D614G) is greater than 95% as determined by SEC-HPLC. The purity of SARS-COV-2 Spike S1 (D614G) is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...