• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to release L-arginine from Benzoyl-Ala-Arg, with detection of the arginine amino group by o-phthaldialdehyde. The specific activity is >40,000 pmol/min/ug.
  • Amino Acid Range: Leu18-Ser423
Catalog #CPM-HM101
Price
Regular price $33000
Regular price Sale price $33000
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Human CPM Protein (CPM-HM101) Summary

Product Description

 Recombinant Human CPM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Ser423.

Product Background

 Carboxypeptidase M (CPM) is a glycosylphosphatidylinositol anchored enzyme that plays an important role in the kallikrein-kinin system (KKS). CPM catalytic domain hydrolyzes Arg from C-terminal peptides (i.e., bradykinin and kallidin), generating des-Arg-kinins, the agonists of B1 receptor (B1R).

Human CPM Protein (CPM-HM101) Specifications/Details

Protein
 CPM
Synonyms
 Carboxypeptidase M; 1110060I01Rik; AA589379
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu18-Ser423
Molecular Weight
 The protein has a predicted MW of 47.48 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to release L-arginine from Benzoyl-Ala-Arg, with detection of the arginine amino group by o-phthaldialdehyde. The specific activity is >40,000 pmol/min/ug.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human CPM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CPM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CPM is greater than 95% as determined by SEC-HPLC. The purity of Human CPM is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...