• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gln19-Pro224
Catalog #CRP-HM101
Price
Regular price $69.30
Regular price Sale price $69.30
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Size
Avantor VWR Fisher Scientific

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Human C-Reactive Protein /CRP Protein (CRP-HM101) Summary

Product Description

 Recombinant Human C-Reactive Protein /CRP Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln19-Pro224.

Product Background

 C-reactive protein (CRP) is a polypeptide molecule belonging to the family of pentraxins. CRP is synthesized primarily by the liver in response to certain pro-inflammatory cytokines. It plays an important role in innate immunity, opsonization by its properties, complement activation and immunoglobulins receptor binding. CRP is a protein of the acute systemic inflammation and is, therefore, a prime marker of inflammation.The CRP is quantified by immunonephelometry or immunoturbidimetry.

Human C-Reactive Protein /CRP Protein (CRP-HM101) Specifications/Details

Protein
 C-Reactive Protein/CRP
Synonyms
 C-reactive protein; CRP; PTX1; MGC88244; pentraxin 1; PTX1MGC149895
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln19-Pro224
Molecular Weight
 The protein has a predicted MW of 24.14 kDa. Due to glycosylation, the protein migrates to 25-27 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM Tris, 300mM NaCl (pH 8.0). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human CRP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CRP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CRP is greater than 95% as determined by SEC-HPLC. The purity of Human CRP is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...