• Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
  • Activity: Immobilized Human DKK1 C terminal Domain, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Met178-His266
Catalog #DKK-HM31C
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Human DKK1 C terminal Domain Protein (DKK-HM31C) Summary

Product Description

 Recombinant Human DKK1 C terminal Domain Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Met178-His266.

Product Background

 Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.

Human DKK1 C terminal Domain Protein (DKK-HM31C) Specifications/Details

Protein
 DKK1
Synonyms
 Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met178-His266
Molecular Weight
 The protein has a predicted MW of 35.78 kDa. Due to glycosylation, the protein migrates to 45-53 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human DKK1 C terminal Domain, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test).
Product Tag
 C-mFc
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human DKK1 C terminal Domain, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test). Immobilized Human DKK1 C terminal Domain, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test).
Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human DKK1 C terminal Domain is greater than 85% as determined by SEC-HPLC. The purity of Human DKK1 C terminal Domain is greater than 85% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...