• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus E-selectin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA.
  • Amino Acid Range: Trp22-Pro554
Catalog #ESE-CM101
Price
Regular price $346.50
Regular price Sale price $346.50
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus E-selectin/CD62E Protein (ESE-CM101) Summary

Product Description

 Recombinant Cynomolgus E-selectin/CD62E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp22-Pro554.

Product Background

 E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Cynomolgus E-selectin/CD62E Protein (ESE-CM101) Specifications/Details

Protein
 E-Selectin/CD62E
Synonyms
 CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Trp22-Pro554
Molecular Weight
 The protein has a predicted MW of 59.47 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus E-selectin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus E-selectin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA. Immobilized Cynomolgus E-selectin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA.
Cynomolgus E-selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus E-selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus E-selectin is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus E-selectin is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...