Sensorgram showing the response of a ligand binding experiment over time. The x-axis represents time in seconds, and the y-axis shows the response in RU (response units). Multiple curves, each representing a different concentration, show the binding kinetics, with varying levels of response reaching a plateau.
  • Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 13.05 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Leu22-Asp369
Catalog #FGF-HM2R4
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Size
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Human FGFR4 Protein (FGF-HM2R4) Summary

Product Description

 Recombinant Human FGFR4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu22-Asp369.

Product Background

 Fibroblast growth factor receptor 4 (FGF R4), also known as CD334, is a 110 kDa glycosylated transmembrane receptor tyrosine kinase.Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human FGFR4 Protein (FGF-HM2R4) Specifications/Details

Protein
 FGFR4
Synonyms
 CD334; FGF R4; FGFR4; FGFR-4; MGC20292; JTK2; TKF
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu22-Asp369
Molecular Weight
 The protein has a predicted MW of 65.3 kDa. Due to glycosylation, the protein migrates to 80-95 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 13.05 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human FGFR4, hFc Tag captured on CM5 Chip via Protein A can bind Human Beta Klotho, His Tag with an affinity constant of 13.05 nM as determined in SPR assay (Biacore T200). Human FGFR4, hFc Tag captured on CM5 Chip via Protein A can bind Human Beta Klotho, His Tag with an affinity constant of 13.05 nM as determined in SPR assay (Biacore T200).
Human FGFR4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human FGFR4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of-Human FGFR4 is greater than 95% as determined by SEC-HPLC. The purity of-Human FGFR4 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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