• Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human LAG3, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA.
  • Amino Acid Range: Asp64-Asn305
Catalog #FGL-HM211
Price
Regular price $34650
Regular price Sale price $34650
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Size
Avantor VWR Fisher Scientific

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Human FGL1 Protein (FGL-HM211) Summary

Product Description

 Recombinant Human FGL1 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Asp64-Asn305.

Product Background

 Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.

Human FGL1 Protein (FGL-HM211) Specifications/Details

Protein
 FGL1
Synonyms
 FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand;HP041; LFIRE1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp64-Asn305
Molecular Weight
 The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LAG3, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA.
Product Tag
 N-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human LAG3, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA. Immobilized Human LAG3, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA.
Human FGL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FGL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LTBR is greater than 90% as determined by SEC-HPLC. The purity of Human LTBR is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...