• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FOLR2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FOLR2 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Thr17-Met228
Catalog #FOL-HM1R2
Price
Regular price $16500
Regular price Sale price $16500
/
Size
Avantor VWR Fisher Scientific

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Human FOLR2 Protein (FOL-HM1R2) Summary

Product Description

 Recombinant Human FOLR2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr17-Met228.

Product Background

 FOLR1 and FOLR2 are equipped with cellular glycosylphosphatidylinositol (GPI) anchors. FOLR1 is secreted from epithelia with or without a micelle-encapsulated GPI-anchor into milk and other body fluids/secretions, e.g. semen where its interaction with spermatozoa indicates a role in male fertility. FOLR1 and FOLR2 serve as serum biomarkers of various diseases. FOLR3 possesses no GPI-anchor and originates from secretory granules of neutrophil granulocytes; its concentration in serum correlates to the FOLR3 content in leukocytes and rises with increased leukocyte counts (infection, malignancy and pregnancy).

Human FOLR2 Protein (FOL-HM1R2) Specifications/Details

Protein
 FOLR2
Synonyms
 FBP;FOLR2;OLR2;BETA-HFR;BETA HFR; FR-BETA;FR BETA; FR-P3; FR P3; β-HFR; β HFR; FR-β; FR β;b-HFR; b HFR; FR-b; FR b
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Thr17-Met228
Molecular Weight
 The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human FOLR2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FOLR2 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human FOLR2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FOLR2 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). Immobilized Human FOLR2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FOLR2 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
Human FOLR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FOLR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human FOLR2 is greater than 95% as determined by SEC-HPLC. The purity of Human FOLR2 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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