• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
Catalog #FRN-CM101
Price
Regular price $346.50
Regular price Sale price $346.50
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Size
Avantor VWR Fisher Scientific

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Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101) Summary

Product Description

 Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from HEK293 with His tag at the C-Terminus of FCGRT.It contains Ala24-Ser297 (FCGRT) and Ile21-Met119 (B2M).

Product Background

 The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.

Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101) Specifications/Details

Protein
 FcRn
Synonyms
 FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Accession
Species
 Cynomolgus/Rhesus macaque
Biotinylated
 no
Amino Acid Range
 Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
Molecular Weight
 The protein has a predicted MW of 31.5 kDa (FCGRT) and 11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT) and 13-15 kDa (B2M) based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM PB, 250mM NaC (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.18 uM as determined in SPR assay (Biacore T200). Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.18 uM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test). Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test).
Cynomolgus/Rhesus macaque FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus/Rhesus macaque FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus/Rhesus macaque FcRn is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus/Rhesus macaque FcRn is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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