• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). The affinity constant of 11.70 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Leu72-Leu251
Catalog #FSF-CM115
Price
Regular price $346.50
Regular price Sale price $346.50
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Size
Avantor VWR Fisher Scientific

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Cynomolgus/Rhesus macaque TNFSF15 Protein (active trimer) (FSF-CM115) Summary

Product Description

 Recombinant Cynomolgus/Rhesus macaque TNFSF15 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu72-Leu251.

Product Background

 TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Cynomolgus/Rhesus macaque TNFSF15 Protein (active trimer) (FSF-CM115) Specifications/Details

Protein
 TNFSF15
Synonyms
 TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A; TNF superfamily member 15;VEGI251
Accession
Species
 Cynomolgus/Rhesus macaque
Biotinylated
 no
Amino Acid Range
 Leu72-Leu251
Molecular Weight
 The protein has a predicted MW of 21.52 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). The affinity constant of 11.70 nM as determined in SPR assay (Biacore T200).
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse DR3, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque TNFSF15, His Tag with an affinity constant of 11.70 nM as determined in SPR assay (Biacore T200). Mouse DR3, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque TNFSF15, His Tag with an affinity constant of 11.70 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 20.8ng/ml determined by ELISA. Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 20.8ng/ml determined by ELISA.
Cynomolgus/Rhesus macaque TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Cynomolgus/Rhesus macaque TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 85% and the molecular weight of this protein is around 60-98 kDa as determined by SEC-MALS. The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 85% and the molecular weight of this protein is around 60-98 kDa as determined by SEC-MALS.
Loaded Human DR3, hFc Tag on ProA-Biosensor, can bind Cynomolgus/Rhesus macaque TNFSF15, His Tag with an affinity constant of 1.30 nM as determined in BLI assay (Gator). Loaded Human DR3, hFc Tag on ProA-Biosensor, can bind Cynomolgus/Rhesus macaque TNFSF15, His Tag with an affinity constant of 1.30 nM as determined in BLI assay (Gator).
The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 90% as determined by SEC-HPLC. The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
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