• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: His20-Leu628(GARP) & Leu21-Ser414(Latent TGF Beta 2)
Catalog #GAT-HM402
Price
Regular price $49500
Regular price Sale price $49500
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human GARP&Latent TGF Beta 2 Complex Protein (GAT-HM402) Summary

Product Description

 Recombinant Human GARP&Latent TGF Beta 2 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of GARP.It contains His20-Leu628(GARP) and Leu21-Ser414(Latent TGF Beta 2).

Product Background

 GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.

Non-biotinylated Human GARP&Latent TGF Beta 2 Complex Protein (GAT-HM402) Specifications/Details

Protein
 GARP&Latent TGF Beta 2 Complex
Synonyms
 Garpin; GARP; LRRC32; D11S833E; GARP&Latent TGF B; GARP&TGF B; Cetermin; G-TSF; LAP; TGF-beta-2; TGF Beta 2; GARP&Latent TGF Beta 2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 His20-Leu628(GARP) & Leu21-Ser414(Latent TGF Beta 2)
Molecular Weight
 The protein has a predicted MW of 70.3 kDa (GARP) and 45.53 kDa (Latent TGF Beta 2). Due to enzyme lysis and glycosylation, the protein migrates to 72-77 kDa (GARP), 13 kDa and 46-48 kDa (Latent TGF Beta 2) based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human GARP&Latent TGF Beta 2 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human GARP&Latent TGF Beta 2 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human GARP&Latent TGF Beta 2 Complex is greater than 95% as determined by SEC-HPLC. The purity of Human GARP&Latent TGF Beta 2 Complex is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...