• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.21 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gln20-Pro156
Catalog #GTR-CM101
Price
Regular price $450.00
Regular price Sale price $450.00
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Size
Avantor VWR Fisher Scientific

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Cynomolgus GITR/TNFRSF18 Protein (GTR-CM101) Summary

Product Description

 Recombinant Cynomolgus GITR/TNFRSF18 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln20-Pro156.

Product Background

 GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Cynomolgus GITR/TNFRSF18 Protein (GTR-CM101) Specifications/Details

Protein
 GITR/TNFRSF18
Synonyms
 CD357; GITR; GITR-D; TNFRSF18; AITR
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Gln20-Pro156
Molecular Weight
 The protein has a predicted MW of 15.87 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.21 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Cynomolgus GITR Ligand, His Tag immobilized on CM5 Chip can bind Cynomolgus GITR, His Tag with an affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). Cynomolgus GITR Ligand, His Tag immobilized on CM5 Chip can bind Cynomolgus GITR, His Tag with an affinity constant of 0.21 uM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). Immobilized Cynomolgus GITR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).
Cynomolgus GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus GITR is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus GITR is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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