• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human B7-H2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS (C136S, C137S), His Tag with the EC50 of 11.0ng/ml determined by ELISA.
  • Amino Acid Range: Glu21-Leu144(C137S, C138S)
Catalog #ICO-MM102
Price
Regular price $29700
Regular price Sale price $29700
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Size
Avantor VWR Fisher Scientific

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Mouse ICOS/CD278 (C137S, C138S) Protein (ICO-MM102) Summary

Product Description

 Recombinant Mouse ICOS/CD278(C137S, C138S) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Leu144(C137S, C138S).

Product Background

 Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1.

Mouse ICOS/CD278 (C137S, C138S) Protein (ICO-MM102) Specifications/Details

Protein
 ICOS/CD278
Synonyms
 AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850;CRP1
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Glu21-Leu144(C137S, C138S)
Molecular Weight
 The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 27-38 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human B7-H2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS (C136S, C137S), His Tag with the EC50 of 11.0ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human B7-H2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS (C136S, C137S), His Tag with the EC50 of 11.0ng/ml determined by ELISA. Immobilized Human B7-H2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS (C136S, C137S), His Tag with the EC50 of 11.0ng/ml determined by ELISA.
Mouse ICOS (C137S, C138S) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse ICOS (C137S, C138S) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse ICOS (C137S, C138S) is greater than 90% as determined by SEC-HPLC. The purity of Mouse ICOS (C137S, C138S) is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...