Human CD40 Ligand/TNFSF5 Trimer Protein (CDL-HM140)

  • Express System: Expi293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD40 Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met113-Leu261

Catalog: CDL-HM140

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Product Description Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from Expi293 with His tag and Flag tag at the N-terminal.It contains Met113-Leu261.
Product Category Recombinant Protein
Target CD40 Ligand/TNFSF5 Trimer
Synonyms CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Gene/Protein ID P29965
Species Human
Exact Sequence Met113-Leu261
Molecular Weight The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD40 Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD40 Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA.
The purity of Human CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Human CD40 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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