Human CD40 Ligand/TNFSF5 Trimer Protein (CDL-HM140)
Catalog: CDL-HM140
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| Product Description | Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from Expi293 with His tag and Flag tag at the N-terminal.It contains Met113-Leu261. |
| Product Category | Recombinant Protein |
| Target | CD40 Ligand/TNFSF5 Trimer |
| Synonyms | CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand |
| Gene/Protein ID | P29965 |
| Species | Human |
| Exact Sequence | Met113-Leu261 |
| Molecular Weight | The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CD40 Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Flag |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human CD40 Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA. |
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The purity of Human CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC. |
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Human CD40 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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