• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
  • Amino Acid Range: Gln24-Gly161
Catalog #IFN-HM40G
Price
Regular price $13200
Regular price Sale price $13200
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human IFN gamma/IFNG Protein (IFN-HM40G) Summary

Product Description

 Recombinant Human IFN gamma / IFNG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Gly161.

Product Background

 Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.

Non-biotinylated Human IFN gamma/IFNG Protein (IFN-HM40G) Specifications/Details

Protein
 IFN gamma/IFNG
Synonyms
 Interferon-gamma; Interferon-ฮณ; interferon; gamma; IFG; IFI; IFN gamma; IFNฮณ; IFN ฮณ;Interferon-g; IFNg; IFN g
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln24-Gly161
Molecular Weight
 The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80ยฐC for 12 months as supplied from date of receipt.๏ผ›-80ยฐC for 3 months after reconstitution.๏ผ›Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. Immobilized Human IFN gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IFN gamma is greater than 90% as determined by SEC-HPLC. The purity of Human IFN gamma is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...