• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
  • Amino Acid Range: Ile23-Ser328(IL-12B) & Arg57-Ser253(IL-12A)
Catalog #IL1-CM112
Price
Regular price $412.50
Regular price Sale price $412.50
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus IL-12 Protein (IL1-CM112) Summary

Product Description

 Recombinant Cynomolgus IL-12 Protein is expressed from HEK293 with His tag at the C-Terminus of IL-12A.It contains Arg57-Ser253 (IL-12A) and Ile23-Ser328 (IL-12B).

Product Background

 Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

Cynomolgus IL-12 Protein (IL1-CM112) Specifications/Details

Protein
 IL-12
Synonyms
 CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12;IL12B; IL12p70; IL12 subunit p35;Interleukin-12; IL-12; IL 12
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ile23-Ser328(IL-12B) & Arg57-Ser253(IL-12A)
Molecular Weight
 The protein has a predicted MW of 34.76 kDa (IL-12B) and 23.64 kDa (IL-12A). Due to glycosylation, the protein migrates to 45-48 kDa (IL-12B) and 38-44 kDa (IL-12A) based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. Immobilized Cynomolgus IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Cynomolgus IL-12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus IL-12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus IL-12 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus IL-12 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...