• Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA. The affinity constant of 3.46 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Tyr37-Asp193
Catalog #IL1-HE018B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human IL-18 Protein (Primary Amine Labeling) (IL1-HE018B) Summary

Product Description

 Recombinant Biotinylated Human IL-18 Protein (Primary Amine Labeling) is expressed from E.coli without tag.; It contains Tyr37-Asp193.

Product Background

 Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.

Biotinylated Human IL-18 Protein (Primary Amine Labeling) (IL1-HE018B) Specifications/Details

Protein
 IL-18
Synonyms
 IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1; IL-1γ; IL-1 γ;IL1 gamma; IL1g; IL1γ; IL1 γ;IL-1 g;Interleukin-18; Interleukin 18; IL-1; IL 1; IL1
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Tyr37-Asp193
Molecular Weight
 The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA. The affinity constant of 3.46 nM as determined in SPR assay (Biacore T200).
Product Tag
 No Tag
Fluorophore
 Biotin-labeled
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-18 R1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Biotinylated Human IL-18, No Tag with an affinity constant of 3.46 nM as determined in SPR assay (Biacore T200). Human IL-18 R1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Biotinylated Human IL-18, No Tag with an affinity constant of 3.46 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA. Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA.
Biotinylated Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-18 is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Human IL-18 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...