• Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-1R2, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 9.2 ng/ml determined by ELISA.
  • Amino Acid Range: Ala117-Ser269
Catalog #IL1-HE40BB
Price
Regular price $742.50
Regular price Sale price $742.50
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human IL-1 Beta/IL-1F2 Protein (IL1-HE40BB) Summary

Product Description

 Recombinant Biotinylated Human IL-1 Beta/IL-1F2 Protein is expressed from E.coil with His tag and Avi tag at the C-Terminus.; It contains Ala117-Ser269.

Product Background

 Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.

Biotinylated Human IL-1 Beta/IL-1F2 Protein (IL1-HE40BB) Specifications/Details

Protein
 IL-1 Beta/IL-1F2
Synonyms
 Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B ;Interleukin-1 β; IL-1 β; IL-1β; IL-1 β;IL1 beta; IL1BETA; IL1β; IL1 β;Interleukin-1 b; Interleukin-1b; IL-1 b
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ala117-Ser269
Molecular Weight
 The protein has a predicted MW of 20.1 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human IL-1R2, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 9.2 ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-1R2, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 9.2 ng/ml determined by ELISA. Immobilized Human IL-1R2, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 9.2 ng/ml determined by ELISA.
Biotinylated Human IL-1 Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-1 Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-1 Beta is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IL-1 Beta is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...