• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA. The affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)
Catalog #IL1-HM1R5
Price
Regular price $33000
Regular price Sale price $33000
/
Size
Avantor VWR Fisher Scientific

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Human IL-15RA&IL-15 Protein (IL1-HM1R5) Summary

Product Description

 Recombinant Human IL-15RA&IL-15 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).

Product Background

 Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.

Human IL-15RA&IL-15 Protein (IL1-HM1R5) Specifications/Details

Protein
 IL-15RA&IL-15
Synonyms
 IL-15 R alpha;CD215;IL15RA;IL-15RA;IL-15R-alpha1;Interleukin-15;IL-15;IL15; Nanrilkefusp alfa;IL15R alpha; IL15R-alpha1;IL 15; Interleukin 15
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)
Molecular Weight
 The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA. The affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80ยฐC for 12 months as supplied from date of receipt.๏ผ›-80ยฐC for 3 months after reconstitution.๏ผ›Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA. Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA.
Human IL-15RA&IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-15RA&IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
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