Human IL-13 Protein (IL1-HM213)

Express System: HEK293
Product Tag: N-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly21-Asn132

Catalog #IL1-HM213

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$264.00

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Human IL-13 Protein (IL1-HM213) Summary

Product Description	Recombinant Human IL-13 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Gly21-Asn132.
Product Background	Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	IL-13
Synonyms	IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
Accession	AAK53823
Species	Human
Biotinylated	no
Amino Acid Range	Gly21-Asn132
Molecular Weight	The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
Product Tag	N-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-13, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4 ng/ml determined by ELISA (QC Test).
	Immobilized Human IL-13, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 7.1 ng/ml determined by ELISA.
	Human IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human IL-13, hFc Tag on ProA-Biosensor can bind Human IL-13Ra1, His Tag with an affinity constant of 1.15 µM as determined in BLI assay .
	The purity of-Human IL-13 is greater than 95% as determined by SEC-HPLC.

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