• Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gly21-Asn132
Catalog #IL1-HM213
Price
Regular price $26400
Regular price Sale price $26400
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Size
Avantor VWR Fisher Scientific

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Human IL-13 Protein (IL1-HM213) Summary

Product Description

 Recombinant Human IL-13 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Gly21-Asn132.

Product Background

 Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

Human IL-13 Protein (IL1-HM213) Specifications/Details

Protein
 IL-13
Synonyms
 IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789;Interleukin-13; IL 13
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly21-Asn132
Molecular Weight
 The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
Product Tag
 N-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-13, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 uM as determined in SPR assay (Biacore T200). Human IL-13, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4 ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4 ng/ml determined by ELISA (QC Test).
Immobilized Human IL-13, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 7.1 ng/ml determined by ELISA. Immobilized Human IL-13, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 7.1 ng/ml determined by ELISA.
Human IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human IL-13, hFc Tag on ProA-Biosensor can bind Human IL-13Ra1, His Tag with an affinity constant of 1.15 µM as determined in BLI assay . Loaded Human IL-13, hFc Tag on ProA-Biosensor can bind Human IL-13Ra1, His Tag with an affinity constant of 1.15 µM as determined in BLI assay .
The purity of-Human IL-13 is greater than 95% as determined by SEC-HPLC. The purity of-Human IL-13 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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