Human IL-1 Rrp2/IL-1 R6 Protein (IL1-HM2L2)

Human IL-1 Rrp-2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1F10, His Tag with an affinity constant of 35.38 uM as determined in SPR assay (Biacore T200).

Immobilized Human IL-1 Rrp-2, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-1 Rrp-2 Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA (QC Test).

Human IL-1 Rrp2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-1 Rrp2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-1 Rrp-2, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-1 Rrp-2 Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA (QC Test). The affinity constant of 35.38 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Asp20-Arg335

Catalog #IL1-HM2L2

Price

$297⁰⁰

Size

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Human IL-1 Rrp2/IL-1 R6 Protein (IL1-HM2L2) Summary

Product Description

Recombinant Human IL-1 Rrp2/IL-1 R6 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Asp20-Arg335.

Product Background

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9). IL1RL2 is a receptor for interleukin-36 (IL36A, IL36B and IL36G). After binding to interleukin-36 associates with the coreceptor IL1RAP to form the interleukin-36 receptor complex which mediates interleukin-36-dependent activation of NF-kappa-B, MAPK and other pathways.

Human IL-1 Rrp2/IL-1 R6 Protein (IL1-HM2L2) Specifications/Details

Protein	IL-1 Rrp2/IL-1 R6
Synonyms	IL-1 R6; IL-1 Rrp2; IL1RL2; IL1Rrp2; IL1RRP2; IL-36R;IL1R6; IL36R
Accession	Q9HB29-1
Species	Human
Biotinylated	no
Amino Acid Range	Asp20-Arg335
Molecular Weight	The protein has a predicted MW of 62.6 kDa. Due to glycosylation, the protein migrates to 75-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-1 Rrp-2, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-1 Rrp-2 Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA (QC Test). The affinity constant of 35.38 uM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human IL-1 Rrp-2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1F10, His Tag with an affinity constant of 35.38 uM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-1 Rrp-2, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-1 Rrp-2 Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA (QC Test).
	Human IL-1 Rrp2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-1 Rrp2 is greater than 95% as determined by SEC-HPLC.