• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
  • Amino Acid Range: Ser21-Glu359
Catalog #IL1-HM4R3B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human IL-1R3/IL-1 RAcP Protein (IL1-HM4R3B) Summary

Product Description

 Recombinant Biotinylated Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser21-Glu359.

Product Background

 IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.

Biotinylated Human IL-1R3/IL-1 RAcP Protein (IL1-HM4R3B) Specifications/Details

Protein
 IL-1R3/IL-1 RAcP
Synonyms
 IL1RAP; IL-1RAP; C3orf13; IL-1 R3; IL-1 RAcP;IL1R3; IL1RAcP
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ser21-Glu359
Molecular Weight
 The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. Immobilized Biotinylated Human IL-1R3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
Biotinylated Human IL-1R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-1R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-1R3 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IL-1R3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...