• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IL-25, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17RB, hFc Tag (Cat. IL1-HM2RB) with the EC50 of 6.2ng/ml determined by ELISA. The affinity constant of 0.81nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Tyr33-Gly177
Catalog #IL2-HM125
Price
Regular price $34650
Regular price Sale price $34650
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Size
Avantor VWR Fisher Scientific

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Human IL-25/IL-17E Protein (IL2-HM125) Summary

Product Description

 Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr33-Gly177.

Product Background

 IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.

Human IL-25/IL-17E Protein (IL2-HM125) Specifications/Details

Protein
 IL-25/IL-17E
Synonyms
 IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25;IL25;Interleukin 25; IL 25
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Tyr33-Gly177
Molecular Weight
 The protein has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-25, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17RB, hFc Tag (Cat. IL1-HM2RB) with the EC50 of 6.2ng/ml determined by ELISA. The affinity constant of 0.81nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200). Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-25, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17RB, hFc Tag (Cat. IL1-HM2RB) with the EC50 of 6.2ng/ml determined by ELISA. Immobilized Human IL-25, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17RB, hFc Tag (Cat. IL1-HM2RB) with the EC50 of 6.2ng/ml determined by ELISA.
Immobilized Human IL-25, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 4.64ug/ml determined by ELISA. Immobilized Human IL-25, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 4.64ug/ml determined by ELISA.
Immobilized Human IL-25, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-25 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. Immobilized Human IL-25, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-25 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-25 is greater than 90% and the molecular weight of this protein is around 40-60 kDa as determined by SEC-MALS. The purity of Human IL-25 is greater than 90% and the molecular weight of this protein is around 40-60 kDa as determined by SEC-MALS.
Loaded Human IL-25, His Tag on Anti-His-Biosensor can bind Anti IL-25 Antibody, hFc Tag with an affinity constant of 91.60 pM as determined in BLI assay. Loaded Human IL-25, His Tag on Anti-His-Biosensor can bind Anti IL-25 Antibody, hFc Tag with an affinity constant of 91.60 pM as determined in BLI assay.
The purity of Human IL-25 is greater than 90% as determined by SEC-HPLC. The purity of Human IL-25 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
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