• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 6.31 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Val22-Ala196(IL-23 alpha) & Met23-Ser335(IL-12 beta)
Catalog #IL2-MM1AB
Price
Regular price $520.30
Regular price Sale price $520.30
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Size
Avantor VWR Fisher Scientific

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Mouse IL-23 alpha&IL-12 beta Protein (IL2-MM1AB) Summary

Product Description

 Recombinant Mouse IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the N-Terminus of IL-23 alpha.It contains Val22-Ala196(IL-23 alpha)and Met23-Ser335(IL-12 beta).

Product Background

 Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.

Mouse IL-23 alpha&IL-12 beta Protein (IL2-MM1AB) Specifications/Details

Protein
 IL-23 alpha&IL-12 beta
Synonyms
 IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β;IL12 b; IL12b; IL23 alpha&IL12 b; IL23 alpha&IL12b
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Val22-Ala196(IL-23 alpha) & Met23-Ser335(IL-12 beta)
Molecular Weight
 The protein has a predicted MW of 20.8 (Mouse IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Mouse IL-23 alpha)&45-50 (Mouse IL-12 beta) kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 6.31 nM as determined in SPR assay (Biacore T200).
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse IL-23R, His Tag immobilized on CM5 Chip can bind Mouse IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 6.31 nM as determined in SPR assay (Biacore T200). Mouse IL-23R, His Tag immobilized on CM5 Chip can bind Mouse IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 6.31 nM as determined in SPR assay (Biacore T200).
Mouse IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC. The purity of Mouse IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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