• Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ala21-Gln169
Catalog #IL2-MM401B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Mouse IL-2 Protein (IL2-MM401B) Summary

Product Description

 Recombinant Biotinylated Mouse IL-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala21-Gln169.

Product Background

 Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.

Biotinylated Mouse IL-2 Protein (IL2-MM401B) Specifications/Details

Protein
 IL-2
Synonyms
 IL2; T-cell growth factor; TCGF; Aldesleukin;Interleukin-2; Interleukin 2
Accession
Species
 Mouse
Biotinylated
 yes
Amino Acid Range
 Ala21-Gln169
Molecular Weight
 The protein has a predicted MW of 20.1 kDa. Due to glycosylation, the protein migrates to 27-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test).
Product Tag
 N-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test). Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test).
Biotinylated Mouse IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Mouse IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
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