• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: The affinity constant of 98.1nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Cys24-Glu540
Catalog #ILR-HM112
Price
Regular price $231.00
Regular price Sale price $231.00
/
Size
Avantor VWR Fisher Scientific

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Human IL-12 R beta 1/CD212 Protein (ILR-HM112) Summary

Product Description

 Recombinant Human IL-12 R beta 1/CD212 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys24-Glu540.

Product Background

 Human IL12RB1 is an autosomal gene that is essential for mycobacterial disease resistance and T cell differentiation. Lung and T cell IL12RB1 expression is allele-biased, and the extent to which cells express one IL12RB1 allele is unaffected by activation. Furthermore following its expression the IL12RB1 pre-mRNA is processed into either IL12RB1 Isoform 1 (IL12Rβ1, a positive regulator of IL12 responsiveness) or IL12RB1 Isoform 2 (a protein of heretofore unknown function).

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human IL-12 R beta 1/CD212 Protein (ILR-HM112) Specifications/Details

Protein
 IL-12 R beta 1/CD212
Synonyms
 IL-12R-beta-1; IL-12RB1; IL12R; IL12RB; CD212; IL-12R subunit beta-1; IL-12R-BETA1; MGC34454;IL-12R-β-1; IL-12R subunit β-1; IL-12R-β1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Cys24-Glu540
Molecular Weight
 The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 98.1nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200). Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200).
Human IL-12 R beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-12 R beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-12 R beta 1 is greater than 90% as determined by SEC-HPLC. The purity of Human IL-12 R beta 1 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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