• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gly34-Lys516
Catalog #ILR-HM127
Price
Regular price $297.00
Regular price Sale price $297.00
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Size
Avantor VWR Fisher Scientific

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Human IL-27RA/TCCR Protein (ILR-HM127) Summary

Product Description

 Recombinant Human IL-27RA/TCCR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly34-Lys516.

Product Background

 A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, http://tccr.unmc.edu) allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human IL-27RA/TCCR Protein (ILR-HM127) Specifications/Details

Protein
 IL-27RA/TCCR
Synonyms
 IL-27RA; TCCR; IL27RA; IL-27R-alpha; IL27R alpha; WSX-1; WSX1; ZcytoR1; CRL1; IL-27 RA; IL27 RA;IL27R-alpha
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly34-Lys516
Molecular Weight
 The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA (QC Test). Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA (QC Test).
Human IL-27RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-27RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...