• Express System: HEK293
  • Product Tag: C-hFc (IgG1)-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.77ug/ml determined by ELISA.
  • Amino Acid Range: His16-Thr228(IL-22R alpha 1) & Met20-Ser220(IL-10R beta)
Catalog #ILR-HM5ABB
Price
Regular price $660.00
Regular price Sale price $660.00
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human IL-22R alpha 1&IL-10R beta Protein (ILR-HM5ABB) Summary

Product Description

 Recombinant Biotinylated Human IL-22R alpha 1&IL-10R beta Protein is expressed from HEK293 with hFc (IgG1) tag and Avi Tag at the C-Terminus of IL-22R alpha 1 and IL-10R beta.It contains His16-Thr228 (IL-22R alpha 1) and Met20-Ser220 (IL-10R beta).

Product Background

 IL-22Rα1&IL-10Rβ are receptors for IL-22 and IL-10, mediate the activation of JAK-STAT, MAPK and other signaling pathways, and participate in the process of many inflammatory diseases. IL­-22 Rα1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL­10 Rβ or IL­20 Rβ and the respective ligands is required for signal transduction[1].

Biotinylated Human IL-22R alpha 1&IL-10R beta Protein (ILR-HM5ABB) Specifications/Details

Protein
 IL-22R alpha 1&IL-10R beta
Synonyms
 IL-22R alpha 1; IL-10R beta; IL-22Rα1&IL-10Rβ;IL22R alpha 1; IL10R beta; IL22Rα1&IL10Rβ;IL-22Ra1&IL-10Rb; IL22Ra1&IL10Rb; IL-22R alpha 1&IL-10R beta; IL22R alpha 1&IL10R beta
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 His16-Thr228(IL-22R alpha 1) & Met20-Ser220(IL-10R beta)
Molecular Weight
 The protein has a predicted MW of 52.07 kDa (IL-22R alpha 1) and 48.85 kDa (IL-10R beta). Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.77ug/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.77ug/ml determined by ELISA. Immobilized Human IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.77ug/ml determined by ELISA.
Biotinylated Human IL-22R alpha 1&IL-10R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-22R alpha 1&IL-10R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-22R alpha 1&IL-10R beta is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Human IL-22R alpha 1&IL-10R beta is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...