• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA.
  • Amino Acid Range: His16-Ala228
Catalog #ILR-MM222
Price
Regular price $34650
Regular price Sale price $34650
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Size
Avantor VWR Fisher Scientific

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Mouse IL-22R alpha 1 Protein (ILR-MM222) Summary

Product Description

 Recombinant Mouse IL-22R alpha 1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains His16-Ala228.

Product Background

 Interleukin (IL)-22 is a member of the IL-10 family of cytokines and represents an important effector molecule of activated Th22, Th1, and Th17 cells, as well as Tc-cell subsets, gammadelta T cells, natural killer (NK), and NKT cells. IL-22 mediates its effects via a heterodimeric transmembrane receptor complex consisting of IL-22R1 and IL-10R2 and subsequent Janus kinase-signal transducers and activators of transcription (JAK-STAT) signaling pathways including Jak1, Tyk2, and STAT3.

Mouse IL-22R alpha 1 Protein (ILR-MM222) Specifications/Details

Protein
 IL-22R alpha 1
Synonyms
 IL-22R-alpha-1; IL-22RA1; CRF2-9; IL22R; IL22R1; IL-TIF-R1; zcytoR11;IL22R-alpha-1; IL22RA1
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 His16-Ala228
Molecular Weight
 The protein has a predicted MW of 51.1 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA.
Mouse IL-22R alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse IL-22R alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Mouse IL-22R alpha 1, hFc Tag on ProA-Biosensor can bind Biotinylated Mouse IL-22, His-Avi Tag with an affinity constant of 13.50 nM as determined in BLI assay . Loaded Mouse IL-22R alpha 1, hFc Tag on ProA-Biosensor can bind Biotinylated Mouse IL-22, His-Avi Tag with an affinity constant of 13.50 nM as determined in BLI assay .
The purity of Mouse IL-22R alpha 1 is greater than 95% as determined by SEC-HPLC. The purity of Mouse IL-22R alpha 1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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