• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ITGA2&ITGB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA2 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.
  • Amino Acid Range: Tyr30-Thr1132 (E534K) (ITGA2) acidic tail & Gln21-Asp728(ITGB1) basic tail
Catalog #ITG-HM1AB
Price
Regular price $412.50
Regular price Sale price $412.50
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Size
Avantor VWR Fisher Scientific

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Human Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein (ITG-HM1AB) Summary

Product Description

 Recombinant Human Integrin alpha 2 beta 1(ITGA2&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus of ITGA2.It contains Tyr30-Thr1132 (E534K) (ITGA2) acidic tail and Gln21-Asp728 (ITGB1) basic tail.

Product Background

 The α2β1 integrin, also known as VLA-2, GPIa-IIa, CD49b, was first identified as an extracellular matrix receptor for collagens and/or laminins. It is now recognized that the α2β1 integrin serves as a receptor for many matrix and nonmatrix molecules. It plays a critical role in platelet function and homeostasis.

Human Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein (ITG-HM1AB) Specifications/Details

Protein
 Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer
Synonyms
 α2β1; Integrin alpha A1 beta 1; alpha A1 beta 1; alpha A1; beta 1; ITGA1&ITGB1; ITGA&ITGB; VLA-2, GPIa-IIa, CD49b;Integrin alpha A1 β 1; alpha A1 β 1; β 1;VLA2, GPIa-IIa, CD49b;a2b1; alpha 2 beta 1; Integrin alpha A1 b 1; Integrin alpha A1b1; alpha A1 b 1; alpha A1b1; b 1; b1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Tyr30-Thr1132 (E534K) (ITGA2) acidic tail & Gln21-Asp728(ITGB1) basic tail
Molecular Weight
 The protein has a predicted MW of 126.6 kDa (ITGA2) & 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 100-130 kDa and 140-170 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human ITGA2&ITGB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA2 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human ITGA2&ITGB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA2 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. Immobilized Human ITGA2&ITGB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGA2 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.
Human ITGA2&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ITGA2&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ITGA2&ITGB1 is greater than 95% as determined by SEC-HPLC. The purity of Human ITGA2&ITGB1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...