• Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gln310-Leu399
Catalog #KRN-DE19D
Price
Regular price $26400
Regular price Sale price $26400
/
Size
Avantor VWR Fisher Scientific

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Canine keratin, type I cytoskeletal 19 (310-399) Protein (KRN-DE19D) Summary

Product Description

 Recombinant Canine keratin, type I cytoskeletal 19(310-399) Protein is expressed from E.coli with His tag at the N-Terminus.It contains Gln310-Leu399.

Product Background

 Keratin 19 (K19) belongs to the keratin family of proteins, which maintains structural integrity of epithelia. In cancer, K19 is highly expressed in several types where it serves as a diagnostic marker.

Canine keratin, type I cytoskeletal 19 (310-399) Protein (KRN-DE19D) Specifications/Details

Protein
 Keratin, type I cytoskeletal 19
Synonyms
 Cytokeratin-19; Cytokeratin19; CK-19; CK19; Keratin-19; Keratin19; K19; KRT19
Accession
Species
 Canine
Biotinylated
 no
Amino Acid Range
 Gln310-Leu399
Molecular Weight
 The protein has a predicted MW of 21.84 kDa same as Bis-Tris PAGE result.
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Canine keratin, type I cytoskeletal 19 (310-399) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Canine keratin, type I cytoskeletal 19 (310-399) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Canine keratin, type I cytoskeletal 19 (310-399) is greater than 95% as determined by SEC-HPLC. The purity of Canine keratin, type I cytoskeletal 19 (310-399) is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...