• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human LAIR1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Gln22-His163
Catalog #LAI-HM4R1B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human LAIR1/CD305 Protein (LAI-HM4R1B) Summary

Product Description

 Recombinant Biotinylated Human LAIR1/CD305 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His163.

Product Background

 LAIR1 (leukocyte-associated Ig-like receptor-1, designated CD305) is an approximately 40 kDa type I transmembrane inhibitory glycoprotein belonging to the Ig superfamily.LAIR1 functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation.

Biotinylated Human LAIR1/CD305 Protein (LAI-HM4R1B) Specifications/Details

Protein
 LAIR1/CD305
Synonyms
 CD305; HLAIR1; LAIR1; LAIR-1
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gln22-His163
Molecular Weight
 The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human LAIR1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test).
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human LAIR1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human LAIR1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test).
Biotinylated Human LAIR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human LAIR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human LAIR1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human LAIR1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...