• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.
  • Amino Acid Range: Arg319-Phe541(L452Q, F490S)
Catalog #LCD-VM1BD
Price
Regular price $41250
Regular price Sale price $41250
/
Size
Avantor VWR Fisher Scientific

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SARS-CoV-2 Spike RBD (Lambda C.37) Protein (LCD-VM1BD) Summary

Product Description

 Recombinant SARS-CoV-2 Spike RBD (Lambda C.37) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(L452Q, F490S).

Product Background

 The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein.

SARS-CoV-2 Spike RBD (Lambda C.37) Protein (LCD-VM1BD) Specifications/Details

Protein
 Spike RBD
Synonyms
 S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession
Species
 SARS-COV-2
Biotinylated
 no
Amino Acid Range
 Arg319-Phe541(L452Q, F490S)
Molecular Weight
 The protein has a predicted MW of 26.15 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.
SARS-CoV-2 Spike RBD (Lambda C.37) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS-CoV-2 Spike RBD (Lambda C.37) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-CoV-2 Spike RBD (Lambda C.37) is greater than 95% as determined by SEC-HPLC. The purity of SARS-CoV-2 Spike RBD (Lambda C.37) is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...