• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: The affinity constant of 0.27 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ala22-Gly788
Catalog #LDL-CM101
Price
Regular price $330.00
Regular price Sale price $330.00
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus LDLR Protein (LDL-CM101) Summary

Product Description

 Recombinant Cynomolgus LDLR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala22-Gly788.

Product Background

 The low density lipoprotein receptor (LDLR) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Cynomolgus LDLR Protein (LDL-CM101) Specifications/Details

Protein
 LDLR
Synonyms
 LDLR; FHC; LDL R; LDL receptor; LDLCQ2; FH
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ala22-Gly788
Molecular Weight
 The protein has a predicted MW of 86 kDa, Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.27 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Cynomolgus LDLR, His Tag immobilized on CM5 Chip can bind Cynomolgus PCSK9, His Tag with an affinity constant of 0.27 nM as determined in SPR assay (Biacore T200). Cynomolgus LDLR, His Tag immobilized on CM5 Chip can bind Cynomolgus PCSK9, His Tag with an affinity constant of 0.27 nM as determined in SPR assay (Biacore T200).
Cynomolgus LDLR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus LDLR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus LDLR is greater than 90% as determined by SEC-HPLC. The purity of Cynomolgus LDLR is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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